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In a study with Sprague-Dawley rats, gene expression was evaluated on days 3 and 11 post-fracture.
In the testes of rats, gene expression of LDHA is down-regulated after phthalate exposure in utero (Plummer et al. 2007) and FGF9 plays distinct roles in testis development and male sex determination (Colvin et al. 2001).
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Average pmch−/− rat gene expression from the three experiments is expressed as a percentage of average pmch+/+ gene expression.
Roche Nimblegen Rat Gene Expression array analysis was performed with Affymetrix GeneChip Rat Gene 1.0 ST Array system (Affymetrix, Santa Clara, CA).
However, validation experiments indicated that the rat gene expression data only increased overfitting during training, so these features were removed.
In addition, rat genome annotation lags behind the mouse and human in maturity, confounding a more comprehensive assessment of rat gene expression data.
All human gene expression was normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA copies, and rat gene expression was normalized to β-actin mRNA copies in all samples.
Moreover, rat gene expression data suggest that persistent pregnancy-induced changes in mammary gene expression may account for the protection of parity [ 39].
A recent publication that combined rat gene expression data and a human genetic association study has identified the first genetic risk factor for chronic pain in humans.
The authors performed a multi-dimensional analysis using rat gene expression and human genetic association that identified a new genetic factor affecting susceptibility to chronic pain.
Three separate types of microarray were used to carry out rat gene expression profiling: - Operon Rat v1.0 OpArray™ is a two-colour gene expression system, containing 5,717 oligonucleotides, almost entirely representative for unique well-documented genes.
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