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Some of the rats from each group were also tested for motor stimulation induced by cocaine (5 mg/kg, i.p).
On withdrawal day 1 (WD1) and withdrawal day 45 (WD45), subsets of rats from each group first received a single 6-h extinction session.
After the wound creation, rats from each group were infected with 0.05 ml of the bacterial suspension for a final infection dose of 500 CFU per wound.
Rats from each group (sham and NVH lesion) were injected intraperitoneally with the nonselective muscarinic receptor agonist oxotremorine (0.25 mg/kg; Sigma RBI, Oakville, ON).
Rats from each group were killed at 24 h post-burn to examine plasma aspartate transaminase (AST) and alanine transaminase (ALT) and KCs were isolated.
The rats from each group (n = 7) were killed on days 10 and 30 and their testes were taken for immunocytochemical evaluation and caspase-3 assay.
Five rats from each group were sacrificed at weeks 4 and 8 post surgery, and a histological analysis was performed on the rats from both groups.
Five rats from each group were used for the immunohistochemical examination.
After administration of the formulations, rats from each group were sacrificed after 2, 4, 6 and 24 h.
The researchers then took eight rats from each group and allowed them to eat as much cocaine as they wanted.
Four rats from each experimental group were maintained individually in metabolic cages for 15 days after surgery, and daily urine specimens were frozen for the urine sample analysis.
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