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A loaded running wheel is not strictly analogous to weight lifting; it's more similar in human terms to a stationary bicycle with the resistance dialed high -- in this case, quite high, as the resistance equaled 30 percent of the rats' body weights in the last week of the monthlong study.
MLSS was performed using overloads of 5.0 7.5% of the rats' body weights.
The rats' body weights were recorded at the start of this study (pre) and at sacrifice (post).
During the experimental period, the rats' body weights were recorded regularly and the doses were modulated accordingly.
Thirty-six male SD rats (body weights: 220 300 g) were kept at a density of 1 rat/cage and placed at an altitude of 400 meters at 22 ± 1°C.
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Male Wistar rats (body weight 150 200 g) were administered, isoprenaline (5 mg/kg, body weight, subcutaneously) alone or along with Ro5-4864 (0.5 and 0.5 mg/kg, body weight, intraperitoneally) once daily for 14 days.
Thirty adult Sprague Dawley rats, body weight of 230 250 g were exposed to simulated altitude ∼7620 m, 282 mm Hg, partial pressure of O2 59 mm Hg for 7 and 14 days continuously in an animal decompression chamber.
Thirty adult male Wistar rats (body weight 300 390 g) were used for the study.
Healthy female Wistar rats (body weight of 200 250 g at start of experiments) were used.
Thirty-four male Sprague-Dawley rats (body weight 254 to 311 g) were anesthetized with an intraperitoneal injection of pentobarbital sodium (5 mg/100 g body weight).
Sixty female Wistar rats (body weight, 150 ± 20 g at study start) were kept in the animal housing facility under standard conditions in the Kiev National University.
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CEO of Professional Science Editing for Scientists @ prosciediting.com