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We also observed high ratios of proline.
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The name of the group refers to the high ratio of proline and glycine amino acid residues found in collagen.
This large difference might be caused by variations in the cis-to- trans ratio of the proline present in synthetic model compounds as a result of differences in the synthetic routes (Fmoc or Boc), as was shown for the peptide DKIHP by Gómez-Ruiz [ 15].
The ratio of phosphorylated proline-rich Akt substrate 40 (PRAS40) which is known to negatively regulate autophagy [ 42, 43] was also not changed in LAMP-2-deficient samples (Additional file 5c, middle panel) indicating no overall increase in basal MA when LAMP-2 is absent.
This transformation corrects for the underestimation of the H/L ratio because of the metabolic labeling of proline and glutamate.
On the other hand, as illustrated in the mutational analysis of cis– trans-proline equilibria in staphylococcal nuclease [ 62], substitutions well removed from the site of proline isomerization can significantly alter the cis/ trans ratio.
The chemical structure of proline is.
Proline metabolism, in terms of proline content (mg g−1 FW), was monitored in the P. vulgaris seedlings (Fig. 2c).
Fig. 5 Change in the concentration of proline and TAC.
Dear Editor, Proline-rich antimicrobial peptides (PrAMPs) are a class of antimicrobial peptides containing a high content of proline residues.
The use of proline as a chiral agent in hydrogenations, where the proline is applied as homogeneous chiral catalyst in ring-closure reactions of triketones, was reported previously.
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