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The percentages were obtained by calculating the ratios of cells with aggregates in ten random viewing field and the presented data were from three independent experiments.
The activation ratios of cells on substrates coated with P-selectin of 0.1, 1.0 and 10 μg/mL at a wall shear stress of 0.2 Pa were 36.5% ± 3%, 56% ± 3%, and 95% ± 4.3%, respectively (Fig. 3B), showing P-selectin concentration-enhanced calcium signaling of neutrophils.
We first performed titration experiments in which PBMCs were incubated with various concentrations of mature P. falciparum-infected erythrocytes (schizonts) to establish the optimal ratios of cells.
Ratios of cells in the G0/G1, S, and G2/M phases of cell cycle were determined on the basis of their DNA content and presented as cell percentage at the end stage of the cell cycle.
Nuclear/cytosol fluorescence intensity ratios of cells were calculated with ImageJ software (Bethesda, MA, USA; http://imagej.nih.gov/ij/, 1997 2012).
Quantitative assessments were based on the morphology preservation of both bacterial cell and surface filaments by comparing the W/ H ratios of cells fixed using different methods.
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The biomimetic polymers consist of a poly vinyl amine) (PVAm) backbone with hexanal branches and varying ratios of cell binding peptide (RGD) to carbohydrate (maltose).
We have considered rounded, square, and hexagonal cell shapes, circular and elliptical nuclei, and different ratios of cell to nuclear size.
Ratios of cell numbers were calculated for the treatment group compared to the controls.
The percentage of apoptotic cells and distribution ratios of cell cycle were determined by flow cytometric analysis.
Log2 ratios of cell line B versus cell line A samples were calculated, local background subtracted, and Loess normalized.
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