Separate feeding (rations 1 and 3) of maize silage in the morning and prewilted grass silage in the evening was compared with a TMR (ration 2).
Rations 1 and 2 provided a normal OEB level (±150 g/day), whereas ration 3 a high OEB level (±400 g/day).
Four lactating ruminal-, duodenand, and ileal-cannulated Danish Holstein dairy cows (143 d in milk, milk yield of 34.3 kg) were submitted to a 4 × 4 Latin square design with 4 rations: 1 control with rapeseed meal (low-fat, CON) and 3 fat-supplemented rations with either rapeseed cake (RSC), whole cracked rapeseed (WCR), or rapeseed oil (RSO).
Forty-eight lactating Danish Holstein cows were used in a 4 × 4 Latin square design experiment with 4 rations: (1) high digestibility and high CP concentration (HdHp), (2) high digestibility and low CP concentration (HdLp), (3) low digestibility and high CP concentration (LdHp), and (4) low digestibility and low CP concentration (LdLp).
Furthermore, urban migrant laborers have great difficulty accessing government programs otherwise accessible in rural settings, including those for health care and insurance [ 4, 54, 55], childcare [ 4, 54, 56], education [ 53, 56], and food rations [ 5, 53, 54].
All RNA samples showed A260/280 ratios between 2.0 and 2.1 and A260/230 rations between 1.8 and 2.1.
The average dry matter intake per kilogram fat corrected milk for rations 1 4 are 0.9, 0.95, 0.96, and 0.95, respectively.
Total mixed rations (250 g/kg of ryegrass and 750 g/kg of concentrate mix) were offered ad libitum.
Plasma glucose concentrations were 51.7, 58.4 and 58.5 ml/100 ml, respectively, in the cows fed the LUDCP, MUDCP and HUDCP rations (Contrast<0.02).
Average daily gain did not differ between the treatment rations, 0.74, 0.81, 0.81, 0.77 kg/d, low to highest CP ME ratio, respectively.
Dry matter intake showed a quadratic effect for the treatment rations, 3.30, 3.41, 3.48, and 3.39 kg/d, low to highest CP ME ratio, respectively, and averaged 2.0% BW.
