The scientific method only works for questions for which we can devise rational experiments to test them against.
Thanks to investors' willingness to take a flyer on things like nanotech, companies are able to do more research and development than is economically rational; they experiment with ideas and approaches that, under leaner conditions, would never be tried.
Consequently, the question that a potential rational design experiment might want to address is which mutation will likely show the most pronounced affect, assuming that a reduction in translation initiation correlates with a smooth transition from the wildtype structure to a conformationally rearranging one without getting trapped in local minima.
Thus, this method helped to more rationally consider the interconnectivity of the chemotactic signalling pathway in R. sphaeroides through relatively simple input-output experiments in vivo and helps to design rational future experiments.
Recent empirical and rational design experiments are beginning to chart this potential new territory in coiled-coil space, leading to intriguing new structures, and possibilities for functionalisation and applications.
Conversely, models built based on low and medium similarity between the target and template sequences can be useful for functional inference, design of rational mutagenesis experiments and molecular replacement in crystallography.
In addition to providing the first structural perspective on mechanism and specificity in HbpA, the structure also reveals new avenues for rational engineering experiments that could be targeted towards improving or altering its activity.
Hoarding and phantom ordering are never rational in the experiment because there is no horizontal competition, randomness, or capacity constraint; further, customer demand is constant and participants have common knowledge of that fact.
This database can also provide guidelines for rational design of experiments to engineer novel polyketides†.
