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The total mixed ration consisted of 800 g/kg corn silage and 200 g/kg concentrate.
The total mixed ration consisted of grass silage, corn silage, soybean meal, and a standard or glucogenic concentrate.
The diet fed as a total mixed ration consisted of 45% corn silage, 5% coarsely chopped wheat straw, and 50% concentrate, on a dry matter (DM) basis.
The total mixed ration consisted of 600 g/kg dry matter (DM) of forage and 400 g/kg DM of concentrate on a DM basis.
Four levels of TA, i.e. 0, 6.5, 13.0 and 26.0 g/kg dry matter (DM), were added to a basal ration consisted of concentrate mixture and corn silage in a 4×4 Latin square design as experimental treatments, respectively.
The total mixed ration consisted of 591.9 g/kg of corn silage and 408.1 g/kg of concentrate [dry matter (DM) basis], and treatments differed with regard to the inclusion or exclusion of PBP as follows: control (CON; excipient without phenolic compounds from the propolis extract), PBP1 PBP3 (3.27, 3.27 and 1.93 mg of phenolic compounds/kg of ingested DM, respectively).
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Cows were fed ad libitum a total mixed ration consisting of equal proportion of forage and concentrate.
Each of the resulting silages was included in a total mixed ration consisting of 35, 55, and 10% (DM basis) of corn silage, concentrate, and alfalfa hay, respectively.
All cows received a basal total mixed ration consisting of 490 g/kg chopped alfalfa hay and 510 g/kg concentrate.
Cows were fed ad libitum a total mixed ration consisting of 200 g/kg corn silage, 200 g/kg alfalfa hay and 600 g/kg concentrate mix.
Treatments were fed as a total mixed ration consisting of 25.4% corn silage, 23.1% grass silage, 11.6% grass hay, and 39.9% concentrate on a dry matter basis.
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