Meanwhile, visualization-based tools should fulfill three requirements [102, 103]: expressiveness (demonstrate exactly the information contained in the data), effectiveness (related to cognitive capabilities of human visual system) and appropriateness (cost-value ratio for visualization benefit assessment).
HER2 positive versus negative status was subjectively determined by ratio plot visualization and objectively determined by calculating the mean of the fluorescence intensity ratio (FIR) values for the HER2 gene probes.
RNA was quantified by UV photometry at 260 nm and the purity and integrity were confirmed by their OD 260/280 ratio and visualization of 18S and 28S rRNA bands on the ethidium bromide gels.
The reconstructed PET/CT images showed positive tracer uptake with a moderate signal-to-noise ratio and visualization of the infected target area-B), that is, right hind muscle tissue.
Excitation with the 360 nm filter (close to the Fura-2 isosbestic point) allowed observation of the cells' morphology and of the changes in the concentration of the dye, irrespective of changes in free cytosolic Ca2+ concentrations ([Ca2+]i), while the 360/380 nm ratio allowed visualization of the [Ca2+]i changes in the cytoplasm.
Moreover, mTOR and S6K1 phosphorylations were elevated in livers of MCD-fed mice in parallel to an increase in the amount of polyubiquitinated proteins, an accumulation of p62, higher LC3-II/LC3-I rando and visualization of punctuate LC3-II staining and autophagosomes compared with CHD controls.
The quality of total RNA extracted was verified spectrophotometrically through A260/A230 and A280/A260 ratios and visualization in agarose gel (1%) with ethidium bromide.
Hierarchical clustering of the log2 ratio matrix, and visualization of the resulting dendrogram, was performed in Partek® Genomics Suite™ 6.4, using Pearson's Dissimilarity to calculate row dissimilarity and Ward's method for row clustering.
This procedure increased the signal-to-noise ratio, allowing the visualization of faint gelatinase bands at 92 , 125and > 200 kDa.
On the MR sialography imaging, the visualization ratio of the ductal system of submandibular gland was evaluated in the cases and volunteers.
