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Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
Central composite rotatable designs were performed as a function of the reaction time (2.5 49.5 h) and the molar ratio of medium chain fatty acid/TAG (MR; 0.6 7.4), for both acids, and also of temperature (32 48 ̊C) for C8 0 experiments.
Figure 5 shows an example of the direction of the minimum variance and the hodogram for an 8-s period from 5 24 0 to 5 24 8, during which the ratio of medium to minimum variance was 4 and the minimum variance direction was calculated to be (0.83, 0.48, –0.28) in SSE coordinates.
During experiments, the original RPMI1640-based culture medium was replaced with the medium (XM) containing 1∶1 ratio of Medium 199 and Ham's F-12 medium, 5% heat-inactivated FBS, and 1 mM glutamine [22].
And this inhibition could be efficiently relieved by regulating the C/N ratio of medium.
Nutrient content of feeds varied significantly, including osmolality, fat composition and ratio of medium chain triglycerides.
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Cells were treated for 5 days with renewal of test compound and media on day 3. On the fifth day, medium was renewed and MTT (3- 4,5-dimethylthiazol-2-yl -2,5-diphenyl-2H-tetrazolium bromide) (Sigma, St Louis, MO, USA) solution (0.5 mg MTT per ml of media) was added to the medium such that the ratio of MTT : medium was 1 : 10.
When decreasing the effective optical contrast ratio of the medium (the ratio of the high refractive to effective refractive index) less than its ordinary value, the relative bandwidth of the 1D-PCs can be changed continuously to any desired value.
The relative composition of C. parriaudii varied depending upon N/P ratio of the medium, with the final proportion of protein and carbohydrate ranging from 5 to 15% and 36 to 54% per unit dry weight, respectively.
This quality improvement orthogonal design array (L27) was used as a mathematical tool to find and study the response prediction of independent as well as significant variables (A = poly-concentration; B = bio-enhancer and C = ratio of organic medium).
We describe the experimental design protocol used to achieve the culture conditions necessary to obtain both high-cell-density and TAG accumulation; specifically, we describe the importance of the C/N ratio of the medium composition.
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