Sentence examples for ratio of insert from inspiring English sources

A 3 1 ratio of insert to vector must be used.

The amount of vector DNA and the ratio of insert to vector DNA were examined.

If the libraries had high ratio of insert fragments and most fragments length were from 500 to 800, they were considered as high quality.

A 1 1 to 3 1 molar ratio of insert:vector yielded highly efficient cloning, with more than 2 × 10 colonies/ng vector being formed (Fig.  2b).

At an approximately 1 10 molar ratio of insert: vector, 150 kb–250 kb DNA fragments were ligated to 30 ng of linearized pBeloBAC11 vector in a 120 μl total volume and incubated at 12°C for 24 48 h.

Before submitting to Illumina for sequencing, the quality of the library was determined by cloning a portion of DNA fragments into the pPCR-Script Amp SK Cloning Vector provided in the PCR-Script Amp Cloning Kit (StrataGene, #211188), per manufacturer's instruction, with the molar ratio of insert to vector DNA ranges at 50 1.

The use, number, and positioning of catheters is determined by the perceived risk-to-benefit ratio of inserting the device(s).

After construction of the cDNA library, the library quality was evaluated by 96-clone test sequencing (Library size, 1.54 × 10; Ratio of insert-including bacterial clone, 79%; Ratio of full-length cDNA insert, 76%; Average of insert length, 0.9 kb).

Consequently, no profound differences were observed from the two different ratios of insert to vector molecules used in the individual ligation reactions.

The SLiCE (JM109) reaction was performed for 60 min at 37 °C with 1 1 and 3 1 molar ratios of insert to vector for Prx IIE and G6PDH1, respectively.

Different molar ratios of insert to vector DNA as well as 1 Weiss unit of T4 DNA Ligase and the supplied buffer from Thermo Fisher Scientific (St. Leon-Rot, Germany) were used.