The amount of sample RNA corresponds to the amount of competitor when the ratio of competitor to target is 1.0.
The amount of the sample RNA corresponds to the amount of competitor when the ratio of competitor to target is 1.0.
4) "Relative fitness was calculated as the ratio of the competitors' growth rates during competition".
For example, at the Olympics at which Didrikson won her two gold medals in 1932, only 127 women took part across all sports - a ratio of female competitors to male of one to 10.
For each microcosm and block, we calculated the selection rate coefficient per day (s) of the small colony morph as the linear regression of the natural logarithm of the ratio of both competitors over time [53].
Proviral LTR sequences were PCR-amplified and sequenced with the-21M13 Big Dye Primer cycle sequencing kit (ABI) to determine the ratio of both competitors [12], [33], [80] and subsequently calculate relative fitness.
In addition, QuantumRNA™ 18S internal control standards (Ambion Inc., Huntingdon, UK), which produced a PCR product of 489 bp, were used at a ratio of 10 1 competitors to primers, in accordance with the manufacturer's instructions, to act as an internal loading control to normalize between samples during densitometry.
The ratio of the two competitors was quantified at the initial and final time points by flow cytometry (Accuri, San Jose, CA). 3 to 6 independent replicates for each fitness measurement were performed.
The ratio of the two competitors was quantified at both the initial and final (72 hr) time points by microscopic visualization of 10 fields of view using a Zeiss Inverted Microscope (Axio Observer Z1) fitted with an AxioCam HR.
Next, we computed a competition ratio (sum scaled confidence rate of competitor +1 divided by the sum scaled confidence rate of target +1) for each gate and type of word.
