Owing to its high signal-to-noise ratio, labeling with BCN revealed fine, subcellular details, which can discriminate surface glycan distribution states on individual living cells as shown by densitometry experiments (see the Supporting Information).
It is concluded that the ratio of label captured from specifically labelled [14C]glucose is a valid and convenient measure of the relative rates of oxidation of the different positional carbon atoms within the supplied respiratory substrate.
Partial labelling seems unlikely, since the molar ratio of label to protein is approx. 15:1, and MIANS is highly reactive.
Epithelium proliferation, as indicated by the labeling index (the ratio of labeled nuclei to total nuclei in the crypt) is 15.6±1.7 in duodenal ulcer and 17.8±1.5 in duodenitis.
Slight delays on the kinetics of Aβ42 were observed due to the addition of labeled peptide regardless of the molar ratio of labeled peptide to unlabeled peptide in the studied range.
In order to study method linearity and working range for each analyte, calibration curves were constructed by plotting the peak area ratio (native/isotopically labelled) versus the mass fraction ratios (native/isotopically labelled), obtained from standard solutions.
In order to standardize the labelling by PTK2BpY402 to the loss of MAP2A labelling in dendrites of 10-12 mo-old Tg mice, we examined the ratio of labelling (pixel density) by anti-PTK2BpY402 thathat of anti-MAP2A.
By contrast, the availability of labeled RBC had little effect on the final parasitemia, as varying the ratio between labeled and unlabeled cells showed only a minor impact on the final parasitemia (Fig. 4C).
We observed that a significantly higher ratio of labeled NG2 was endocytosed and recycled to the membrane in Lgl1 cKO cells compared to WT cells (WT = 15%; cKO = 58%, p < 0.0001) (Figs. 7e g).
Another powerful finding in cluster ensemble research is that the blind criterion Averaged Normalized Mutual Information seems to replace actual misclassification ratio, whenever labels are given to actual clusters.
