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Whole heart cell lysates (n = 3) were analyzed using a Bioassay Systems Enzylight™ ATP ADP ratio kit (ELDT-100, Bioassay Systems, Hayward, California).
ADP and ATP levels were measured using an ADP/ATP ratio kit (Abcam).
ADP/ATP ratios were determined using the fluorescent ApoSensor ADP/ATP Ratio kit (BioVision).
Novaquant Mouse Mitochondrial to Nuclear DNA Ratio Kit (Novagen) was used as per manufacturer's directions, using 1 ng of isolated genomic DNA.
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ADP/ATP ratios were determined using the EnzyLight™ ADP/ATP ratio assay kit from BioAssay Systems (Hayward, CA, USA) according to the manufacturer's instructions.
ADP/ATP ratios were determined using the Enzylight ADP/ATP ratio assay kit (BioAssay, Hayward, CA, USA), according to the manufacturer's instructions.
Intracellular levels of ATP and ADP were quantified with the ApoSensor ADP/ATP ratio assay kit from Bio Vision (Mountain View, CA).
Total amounts of cellular GSH were determined using the GSH/GSSG (GSSG, oxidised glutathione) ratio assay kit (Calbiochem-Novabiochem, San Diego, CA, USA) following the manufacturer's protocol.
Supernatant ATP and ADP contents were measured by the EnzyLight™ ADP/ATP Ratio assay kit (BioAssay Systems, Hayward, CA, USA, ELDT-100) according to the manufacturer's instructions.
The ApoGlow adenylate nucleotide ratio Assay Kit (Cambrex Bioscience, Nottingham, UK) was used to discriminate between inhibition of proliferation, induction of apoptosis and cell lysis by determination of ADP: ATP ratios, using the luciferin-luciferase bioluminescence assay and in accordance with the manufacturer's instructions.
NAD + /NADH or NADP + /NADPH levels were measured with Amplite™ Fluorimetric NAD/NADH or NADP/NADPH ratio assay kits (ATT Bioquest, CA) as previously described [ 17].
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