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Interobserver agreement of the rating of staining intensity showed a moderate agreement (Fleiss' kappa was 0.44).
Rating of staining intensity and positive tumor ratio was repeated on the scanned slides, utilizing our viewer program.
Second, while we found that the reproducibility of positive tumor ratio estimation does not profit from experience, the rating of staining intensities does.
There was also a significant difference in favor of calcitriol ointment in the rating of staining and irritation (P < 0.01) and a higher proportion in the calcitriol group rated the overall acceptability of treatment as good (47%) than in the dithranol group (20%).
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The absorbance of the final reaction product was measured at 660 nm (A660) and was converted to the rate of staining (ΔA660·µm−1 section thickness·s−1 incubation time), where ΔA660 is the change in absorbance at 660 nm).
We rated the intensity of staining on a scale of 0 to 2: 0, negative; 1, weak; and 2, strong.
P16INK4a produced an unexpectedly high positive rate of staining in the low-risk group (60%).
Its rate of staining of rhabdomyosarcomas is compared with the results in the literature.
In the third round of rating we first repeated the estimation of staining intensities with the viewer program.
Likewise, the proliferation rate was evaluated with the help of staining for Ki-67.
According to the age-adjusted Kaplan Meier analysis performed, the survival and DFS probability rates dropped with the TUNEL level of staining.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com