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Some PCR failures could result from the loss of genetic regions rather than primer problems.
Rather than primer conditions, divergence of functionally important genes might be the cause of low cross-species amplification success of the gene-based SSRs.
An earlier report using a PCR rather than primer extension assay found that long extension products were Est1p dependent, perhaps providing additional evidence for an activating role for this subunit (Lin and Zakian 1995).
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It not only stood for the idea that reading ought to be taught by phonics; it also stood for the idea that language skills — and many other subjects — ought to be taught through illustrated storybooks, rather than primers and textbooks.
It not only stood for the idea that reading ought to be taught by phonics; it also stood for the idea that language skills and many other subjects—ought to be taught through illustrated storybooks, rather than primers and textbooks".
Thus we will typically refer to multiplexing SNPs (rather than primers) but our treatment is readily applicable to most other PCR applications.
First, we can choose a T7 phage library that was constructed using oligo-dT primer rather than random primers.
Viral primers were designed to cross gene boundaries so only viral genomes were quantified (rather than mRNA); primer sequences are shown in Table S2.
The follow-your-nose quality of the book – it's definitely a personal journey rather than a primer – has advantages and disadvantages.
An example of industrialization relates to the current manufacturing of oligonucleotide primers used all over the world for polymerase chain reaction (PCR): nowadays most researchers order primers from centralized companies rather than synthesizing primers at local laboratories, mainly because these companies have achieved higher quality at a reduced price.
Therefore, it is most convenient to pin replicate the DNA templates, rather than the primers.
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CEO of Professional Science Editing for Scientists @ prosciediting.com