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Cell proliferation rates were assayed using the Cell Counting Kit-8 (Dojindo Laboratories, Japan) according to the manufacturer's instructions.
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The samples were assayed using the plaque forming technique.
Enzyme activity was assayed using 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate
Qu was assayed using an HPLC method.
Creatinine was assayed using a spectrophotometric assay.
Resistance rates were determined using fluctuation assays essentially as in Srivastava et al. (2012).
The proliferation rates were evaluated using MTT assays at 3, 5 and 6 days post seeding.
HIV incidence rates were calculated using the BED assay results with a cut-off value of 1.89, which corresponds to an assay window period of about 15 months, and with correction for misclassifications according to a published method [ 21].
Substrates OAA and AcCoA were both added at 100 μM and initial reaction rates were measured using the DTNB assay described above.
ATPase rates were determined using an NADH-coupled assay as previously described (Patel et al., 2011).
LDL adsorption and desorption rates were estimated using an enzyme-linked-immunosorbent assay, which revealed that the obtained anionic glycosylated PSf membrane had a higher affinity for LDL than either glycosylated or sulfonated membranes alone.
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