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Feed intake, milk production, body weight, and body condition score were monitored, and linear and quadratic effects of increasing the alfalfa inclusion rate were assessed using mixed model analysis.
The effects of acute administration of prucalopride, either on the fluvoxamine- and citalopram-induced inhibition of 5-HT neuron activity, or on hippocampal neuron firing rate, were assessed using a within-design one-way ANOVA, followed by Tukey's post hoc test.
Differences in intervention rate were assessed using negative binomial regression modeling.
At a macrostructural level, the effects of strain (SHR vs. WKY), extinction session (EXT1 vs. EXT2), and time in extinction (eight 8.125-min 8.125-minach session) on log response rate were assessed using a mixed-design ANOVA.
The effects of TGF-β1 signalling on the cell growth rate were assessed using cells transfected with a TGF-β1-expression vector/silencing vector or following pre-incubation in serum-free medium in the presence or absence of TGF-β1 (5 ng/ml) or anti-TGF-β1 antibody (10 μg/ml) for 48 h.
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After 24 or 48 h incubation, apoptotic rate was assessed using a Cell Death Detection ELISA kit.
Lapse rate was assessed using a verbal letter identification paradigm like that used in visual acuity and letter contrast sensitivity testing.
The influence of the variables on the degradation rate was assessed using the experimental results obtained from a full factorial 23 experimental design.
The surface hardness is obtained using hardness tester, phase composition is evaluated by X-ray diffraction, surface features are observed using scanning electron microscope (SEM) along with energy-dispersive X-ray spectroscopy (EDS) and wear rate is assessed using the ball-on-disc tester.
The cells were then detached using trypsin, and the survival rate was assessed using MTT assays.
5-HT synthesis rate was assessed using the decarboxylase inhibition method.
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