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Apoptosis rate was detected using the Annexin V-FITC detection kit (Beyotime) according to the manufacturer's instructions.
Then, apoptosis rate was detected by FITC Annexin V Apoptosis Detection Kit I (BD Pharmingen) according to the user's manual in BD FACS Aria I. Apoptotic ratio in the silenced groups was compared to the negative control siRNA transfected cohort.
The apoptosis rate was detected using the in situ cell death detection kit, tetramethylrhodamine red (Roche).
No significant increase in spark rate or strip loss rate was detected and the chambers operated stably.
A significant difference of prevalence rate was detected in depression symptoms between EM and CM (χ2 = 4.05, P = 0.044).
(F) The apoptotic rate was detected with the Annexin V-APC kit following 48 h of suspension culture.
(B) Left: The cell apoptosis rate was detected with flow cytometer in MD-1, MT and control group.
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No significant differences on the adsorption rate were detected.
No significant changes in heart rate were detected.
In the end the count rate due to stray light and the dark count rate is detected.
Post-thaw sperm motility, fertilization and hatching rate were detected to evaluate the reliability of sperm cryopreservation.
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