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In the case of non-bleaching fluorophores the maximum error rate is f2/2 f1 [ 22], while in the case of bleachable fluorophores it is f2/ f1 [ 21] We will normalize the error rate to simplify our notation for p: (4) p = 2 f 1 E 2 / f 2 n ≡ E ˜ n where Ẽ is the normalized error rate Ẽ = 2 f1 E2/ f2.
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