Sentence examples for rate of unwinding from inspiring English sources

Variation of the amount of unwound RNA substrate as a function of time was fitted to a simple exponential equation:

The rate of unwinding is 47 bp/s, identical to that of the wild type for identical junctions.

Involvement of nonconserved residues is necessary for the determination of their roles in the rate of unwinding and determination of key residues for helicase inhibitors.

Therefore, we do not extract this information and simply fit the lag kinetics and exponential rise to determine the average rate of unwinding.

(B ) The base pair unwinding rates of the combined enzymes at 5 µM dVTPs plotted against dTTP concentrations and fit to Equation 4 (solid lines) to obtain the maximal rate of unwinding (k cat) and K m for dTTP.

We show that the dTTP-bound state is slightly better at capturing the DNA-base than the dTTP-free state, but the equilibrium constants of the base-capture steps in the dTTP-free (K1) and dTTP-bound (K2) states both decrease with increasing GC percentage, which indicates that the rate of unwinding by the helicase is limited by base pair separation even at high concentrations of dTTP.

The corresponding progress curves are shown in Fig. 5 C and the calculated apparent rates of unwinding (kuw) are reported in Table 3. Addition of the trap reduced the unwinding rate of DDX3wt by about 2- fold, suggesting that under distributive conditions (i.e. without trap) DDX3wt required two binding events in order to fully displace the 18mer strand from the complementary 36mer.

Therefore, T7 DNAP is able to achieve fast rates of unwinding at high dNTPs, but this is not the case with the helicase, whose unwinding kcat remains suboptimal even at high-dTTP concentrations.

(B ) Relative rate of DNA unwinding by T7 DNAP with increasing concentration of E. coli SSB to determine the optimum concentration of SSB to use in the unwinding experiments.

DNA chain breaks, however, can be detected with great sensitivity by exposing crude cell lysates to alkaline solutions and monitoring the rate of strand unwinding so that one strand break per chromosome can easily be detected.

This step is represented by the single irreversible rate constant (klim) that leads to the covalent E MCsp complex in Figure 4A (the assumption of irreversibility is justified by the rapid rate of supercoil unwinding that follows cleavage, as described below).