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Understanding what drives the rate of disruption is helpful for predicting outcomes, but it doesn't alter the way disruptions should be managed.
In previous columns I have described our current moment of crisis specifically the rise of Trump, the far right, and populist strongmen as the result of two factors: (1) the increasing rate of disruption and (2) the lack of a capacity to lean into these moments by letting go of the old and letting come new patterns and possibilities (a capacity I call presencing).
The chief methodological difficulty has been the increasing rate of disruption of these runs over time by chromosomal rearrangement.
However, it occurred to us that our observed de-adhesion dynamics might also depend on the rate of disruption of cell substrate adhesions (i.e., trypsin/EDTA activity), which might vary across drug treatments and cell lines.
We used a simple 'relative measure' approach to separate the topological and biochemical effect of each disruption by comparing the growth rate of disruption strains in stressful and reference environments.
To estimate the effect of these disruptions on robustness we measured the growth rate of disruption strains, relative to the reference strain, in response to various stresses, both external (environmental) and internal (mutational).
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Importantly, under experimental conditions that allowed similar rates of disruption using LP-directed ZFN delivery and plasmid-encoded ZFN production, off-target activity within the CCR2 locus was detected only after ZFN-encoding plasmid delivery.
The mean effect of random and hub gene disruptions was determined by measuring the growth rate of each disruption strain within these two groups (figure 1).
The overall rate of uterine disruption was 1.1% of all women attempting labor; the rate of true rupture was 0.8%; and the rate of hysterectomy was 0.5%.
Protective skin products are developed, but it is uncertain whether the use of such products is not associated with a higher rate of dressing disruption, which is also a risk factor for CLABSI [1].
Working a rotating shift was linked to a 33percentt greater rate of menstrual disruption and an 80percentt greater rate of sub-fertility.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com