Sentence examples for rate of conjugation from inspiring English sources

Among these, BSA is the most popular one, due to its stability of physical and chemical properties, low price, easy obtainability, and highly successful rate of conjugation.

We must assume then that the rate of conjugation is limited by physical factors and availability of recipient cells rather than the efficiency of conjugation machinery.

Nevertheless, the lack of effect of MK571 treatment on the apical:basolateral ratios for any of the flavonol conjugates confirms that MK571 is not specifically affecting efflux transport of the flavonol conjugates (and in particular MRP-2 mediated efflux to the apical compartment) and that its major effect is to reduce the rate of conjugation of flavonols.

The rate at which the cells generated PpIX (total production, i.e. intracellular and medium), was actually increased by chronic glucose deprivation, suggesting either induction of the rate limiting enzyme, thought to be porphobilinogen deaminase (Gibson et al, 1998), or a reduction in the rate of conjugation to iron to form haem by the enzyme ferrochelatase.

GST activity was quantified by measuring the rate of conjugation of reduced glutathione by GST to CDNB (1-chloro-2,4 dinitrobenzene) at 340 nm and was expressed in nmoles of conjugated CDNB/min/mg protein, using an extinction coefficient of 0.0053 μM-1.

Hence the ratio of conjugation rate to phage infection rate is ∼5, meaning a F+ cell has a higher probability of encountering a F− and conjugating than being infected by a M13 phage within the regime defined by our inoculation conditions.

In this assay, the rate of glutathione conjugation to the substrate CDNB (1-chloro-2,4-dinitrobenzene) is measured.

In brief, GST activity was assayed spectrophotometrically at 340 nm by measuring the rate of CDNB conjugation with GSH as a function of time, according to the method by Habig et al. The assay mixture contained 1 mM GSH and 1 mM CDNB in 0.1 M K-phosphate buffer, pH 6.5.

The assumption of rate limiting nature of conjugation is not arbitrary.

The saturation is also owing to the rate limiting nature of conjugation.

DOI: http://dx.doi.org/10.7554/eLife.04883.005 The regulation of Cry1/2 protein stability is complex and involves differential expression and localization of the E3 ligase subunits Fbxl3 and Fbxl21 that compete for Cry binding and have different rates of ubiquitin conjugation (Hirano et al., 2013; Yoo et al., 2013).