Exact(1)
For determining the rate of cell viability, the MTT [3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide] assay was used.
Similar(59)
beta-5-expressing LECs and controls were analyzed for the expression of beta1, beta2, beta5, and alpha6 proteasome subunits; chymotrypsin-like (CT-L) and peptidylglutamyl-peptide hydrolase (PGPH) catalytic activities; as well as for the accumulation of carbonylated proteins, rates of cell viability, and apoptosis after oxidative stress.
Cell viability in the vitrified cell sheets did not differ between protocols with or without COOH-PLL (92.1% vs. 91.9%); the rates of cell viability were comparable to that of the non-vitrified control (94.6%).
Rat cardiac H9c2 cells were treated with TALA (1, 2, 5, 10, 20 μM) in the absence of DOX for 24 h, and then, the rates of cell viability were evaluated by CCK-8.
Otherwise, RNA based approaches, which would target the active part of a microbial community, thus enabling discrimination between living and dead cells, experience problems with the fast RNA-decay rates after loss of cell viability (Belasco, 1993) and are more expensive and laborious with respect to DNA based analysis.
The development of a fast inverse-electron-demand Diels Alder reaction, which involves the reaction of a trans-cyclooctene with a tetrazine, yields reactions with highly rapid rates and the maintenance of cell viability, which has enabled its use for fluorescent labeling of cell surfaces and intracellular labeling of living mammalian cells.
Log-phase cells were exposed to different concentrations of ZnO NPs for hours, followed by in vitro tests of cell viability, survival rate, morphological changes, apoptosis, and intracellular ROS and reduced glutathione (GSH).
In-vitro cytotoxicity and biocompatibility behaviour of ZrO2 film were investigated using fibroblast cell line, which exhibited high degree of cell viability and proliferation rate rather than bare 316L SS substrate.
The amounts of cell viability, glucose consumption rate, and the yield of sodium gluconate in group C and group D were higher, where more substrates were directly converted to products on the cell wall and less metabolic flux flowed into the cell.
Figure 6 Effect of FMNPs on survival rate of HEK293 cells, HEK293 cells viability measured by CCK8 assay and the percentage of cell viability was calculated as a ratio of OD of FMNPs-treated cells and control cells.
Determination of cell viability.
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