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This result indicates that longer sequences have a higher rate of annotation than shorter sequences.
This rate of annotation is similar to that in sockeye salmon using similar techniques (∼22%; Everett et al. 2012).
The use of the restriction enzyme SbfI, commonly used for RAD sequencing in salmonids, was also intended to increase the rate of annotation (Baird et al. 2008).
The rate of annotation was high with 65% of the 1,339 differentially expressed SuperSAGE tags which could be annotated (57% of which matched known proteins).
The relatively low rate of annotation rediscovery for GO terms based on the text from abstracts related to associated genes is not surprising.
We initially anticipated a much lower rate of annotation consistent with the percentage of protein coding sequence in salmonids (5% c.f. Cheng et al. 2005).
Similar(49)
Similar rates of annotation were reported for transcriptome assemblies of chickpea and red clover [ 48, 49].
Comparably low rates of annotation have been reported for other dinoflagellate EST projects, with only 9% of the (~1, 400) ESTs isolated from Alexandrium ostenfeldii homologous to known proteins [ 48] and ~20% (of 6, 723) of ESTs from Alexandrium tamerense identified [ 21].
Therefore, we directly measured the rate of character annotation for the CA dataset.
The rate of all annotation hits in contigs is predictably higher at 68%, with 42% in singlet sequences.
In order to establish the expected error rate of EC annotation by SPs we perform an analysis of the E. coli genome that contains 4,639,675 nucleotides.
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