Sentence examples for rate freezer from inspiring English sources
For all successive transplantations MSC were cryopreserved in 10% Dimethyl sulfoxide (DMSO) in growth medium using a controlled rate freezer (Kryo 360 3.3, Planer Plc UK).
Cooling rates of (a) 5 °C min−1, (b) 0.1 °C min−1 and (c) direct plunge into liquid nitrogen were set using a Grant Asymptote (EF600) controlled rate freezer.
The cells and tissues were either frozen crushed in the rapid controlled rate freezer (the NSF grant support to MM .or native disintegrated with ultrasonicator (Branson Ultrasonic, Danbury, CT, USA).
The cells and tissues were either frozen crushed in the rapid controlled rate freezer (NSF grant support to MM .or native disintegrated with ultrasonicator (Branson Ultrasonic, Danbury, CT, USA).
The cell concentration per single dose was calculated so the volume of the final dose (3 × 106/kg of patient's body weight) was frozen and stored in 2 mL CellSeal cryogenic vials using a controlled rate freezer and stored in vapor phase of liquid nitrogen.
Cells were cryopreserved by a validated Kryo-16 Controlled Rate Freezer (Planer, Middlesex, UK).
For this purpose, current controlled rate freezers (CRFs) are suitable for controlling temperature, maintaining the rate of temperature during cryopreservation.
In our experiments, we continued to increase DC yields by introducing a computer-assisted controlled-rate freezer (CRF).
After collecting the cells by centrifugation and re-suspension in complete medium, cells were passaged re-plated, loaded into scaffolds, or were frozen using Fisher's CryoMed Controlled-Rate Freezer (Thermo Fisher Scientific, Waltham, MA, USA) and stored in liquid N2.
We present data in favor of using highly concentrated PBMC, cryopreserved by a computer-assisted controlled-rate freezer (CRF), to reach a maximum yield of viable iDC after 6 days of culture.
In contrast to standard isopropyl alcohol (IPA) freezing, PBMC cryopreservation in an automated controlled-rate freezer (CRF) with subsequent thawing and differentiation resulted in significantly higher cell yields of immature and mature DC.
