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The variables determined were: penetration rate, mean number of spermatozoa per oocyte, male pronucleus formation rate, monospermy rate, cleavage rate after 48 h of fertilization, blastocyst rate, and mean number of nuclei per blastocyst.
In comparison with the unmodified substrate (Figure 2), the assemblage of antisense element, substrate and aptamer into a tripartite structure-switching substrate (termed "ASAP") did not allow RNA cleavage to occur with a similar rate, cleavage yield or fluorescence enhancement even under the ATP-saturating condition (Figures 4B and 4C).
A 2′-deoxy or 2′-amine substitution affects substrate binding, cleavage rate, cleavage specificity, and metal binding.
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In order to study the influence of pre-existing dislocations on the pop-in phenomenon in magnesium oxide, an original experimental protocol, based on low-rate cleavage, has been developed to introduce a controlled dislocation density in MgO single crystals.
In addition, our data show that the rate of cleavage at Arg of rP2-II appears to be affected more than the rate of cleavage at Arg of rMZ-II, by prothrombinase assembled with the mutant cofactor molecules.
A comparison of the rate of cleavage of X0n with and without a 5' phosphate shows that the presence of a 5' phosphate increases the cleavage rate by >50-fold (Figure 1E).
The rate of cleavage of rMZ-II by prothrombinase assembled with factor Va5A is 33-fold slower than the rate of cleavage of rMZ-II by prothrombinase assembled with factor VaWT (Table 2).
We have investigated the self-cleavage of gpASNase3 and found that analogously to the human enzyme, gpASNase3 has a very slow intrinsic rate of cleavage.
A number of TEVp mutants with different rate of cleavage, stability and specificity have been reported.
Using MCD-treated spermatozoa, there was no difference in sperm-penetration rate, oocyte cleavage rate, and blastocyst formation rate between the caffeine-free and caffeine-supplemented groups.
Removing the bulge did not significantly slow the rate of cleavage but slowed the conversion of inactive to active conformations.
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