Here, we investigate the rate-breadth correlation using the data on orthologous proteins from Drosophila and Mammalia.
Such sites evolve at above-average rates, due to lack of selective constraint, and all amino acids are acceptable at them, leading to a positive rate-breadth correlation.
Inexperienced academic patenters appear to have obtained patents that proved to be less significant (in terms of the rate and breadth of their subsequent citations) than those issuing to more experienced university patenters.
The magnitude, response rate and breadth of HIV-1 specific T-cell responses in each group and the possible impact of pre-existing Ad5 antibodies on these responses were assessed using an IFN-γ ELISPOT assay.
In order to visibly corroborate the association of bimodal distribution with structural variations, we applied principal component analysis using XSTAT to gene subgroups of low and high GC content with intron size, intron number, evolutionary rate, expression breadth and level as variables.
Rate and breadth are two distinct, and only weakly correlated, characteristics of protein evolution.
Reviewer's report Title: Rate and breadth of protein evolution are only weakly correlated Version: 2 Date: 1 November 2011 Reviewer number: 3 Report form: Review of the paper Rate and breadth of protein evolution are only weakly correlated" by SA Naumenko and AS Kondrashov.
Indeed, on a sample of only 11 or 13 genomes, the "real" values of rate and breadth of evolution are determined, for a particular site, only with substantial sampling errors, which reduces their correlation.
Based on 30 adult human tissues, we defined expression rate and breadth as the mean expression level and the number of tissues in which the gene is expressed, respectively (Waldman et al. 2010).
The strengths of the study include a nationally representative sample with a high participation rate, the breadth of indicators of childhood circumstances and the potential to study their concordance with current circumstances and several indicators of health behaviour.
Reviewer's report Title: Rate and breadth of protein evolution are only weakly correlated Version: 2 Date: 5 October 2011 Reviewer number: 2 Report form: The authors demonstrate that the frequency of amino acid substitutions (rate of protein sequence evolution) and the repertoire of replacements in the substituted sites (breadth of protein sequence evolution) are correlated only weakly.
