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The animals were killed 48 h after surgery and the ventral prostates from each rat were weighted, sliced into pieces and sonicated for 30 min in PBS for bacteria counting.
The parameters of each rat were weighted by the goodness of fit of that model to their data.
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Body weight of each rat was weighted on PND90 and anogenital distance (AGD) was measured using vernier caliper by a single investigator in a blinded manner on the same day; the AGD of each animal was divided by the cube root of body weight (AGD/body weight1/3) as the adjusted AGD to avoid errors caused by differences in body size.
Rats were weighted on P2, P9, P21, and P35 (sacrifice) and brain weights were obtained on P21 or P35.
Rats were weighted and received a subcutaneous injection of buprenorphine 50 μg kg−1.
The brain, liver, spleen, heart and kidney harvested from the rats were weighted individually then examined macroscopically for any abnormality.
Rats were weighted and scored daily according to the following clinical scale: 0 = no clinical signs, 1 = tail weakness, 2 = tail paralysis, 3 = incomplete paralysis of one or two hind legs, 4 = complete hind limb paralysis, 5 = moribund On day 12 post-immunization, EAE rats (n = 14) received an intracerebroventricular (intra-CSF) injection of sterile latex fluorescent microspheres diluted 1/1000.
All rats were weighted once a month and the amount of food consumed was measured.
All the rats were weighted at day 1 and at day 46 using an Acculab V-1200 electronic balance.
At the beginning of the experiment, 22 month-old rats were randomly separated in two groups and were weighted at regular intervals.
The pregnant rats were allowed to deliver spontaneously and at birth pups were weighted and killed by cervical dislocation.
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