The hippocampus isolated from a postnatal day 0 (P0) SD rat was washed twice and mechanically dissociated into single cells by gentle trituration through a Pasteur pipette in aseptic PBS.
RBCs isolated from venous blood of rats were washed with glucose-free phosphate-buffered saline and labeled with 18F-FDG.
For rat embryonic fibroblast (REF) isolation, uteri isolated from 14-day-pregnant SD rats were washed with phosphate-buffered saline (PBS).
For rat embryonic fibroblast (REF) isolation, embryos from E14.5 timed-pregnant rats were washed with PBS.
Briefly, pooled brain tissues without cerebella of four rats were washed with ice-cold buffer and their weight was measured.
Freshly isolated rat cells were washed trice with PBS (4°C), and soluble protein was extracted in 50 µl 0.5% (w/v) RapiGest detergent in 50 mM ammonium bicarbonate (Waters Corporation, Milford, MA). in the presence of Complete Protease Inhibitor Cocktail (F.
Final blood samples (3 mL) were collected in heparin-buffered tubes, rat brains were washed with ice-cold water and homogenized in 0.9 % aqueous NaCl solution (1 mL) using an IKA T10 basic Ultra-turrax (IKA Laboratory Equipment, Staufen, Germany).
Microtubules were depolymerized in 33 µM nocodazole (Calbiochem, Darmstadt, Germany) in MHM for 30 minutes at 37°C, and then cultured APs isolated from the wild-type and rSey /rSey rat embryos were washed with MHM and incubated at 37°C to allow for regrowth.
B103 rat neuroblastoma cells were washed with PBS containing 1 mM CaCl2 and 1 mM MgCl2 and fixed for 10 min with 3.5% paraformaldehyde.
For picking and passaging, rat iPS cells were washed once with ES medium and then mechanically picked (until passage 10) or incubated with 1 mg/ml collagenase type IV (Stem cell Technology, INC., Vancouver, Canada) for 10 min. An appropriate volume of the medium was added, and the contents were transferred to a new dish on REF feeder cells.
