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Separately, the dark and light samples for each rat were then pooled and volume measured.
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The rat was then isolated in a cage, so that he would not be hurt by his congeners.
The rat was then implanted with a jugular vein catheter.
The target rat was then removed from the T-maze and a new trial started with next target rat.
Each rat was then allowed to find the submerged platform within 90 sec, and rest on it for 20 sec.
The rat was then returned to the cage.
The free rat was then placed in the arena.
The rat was then fixed on a stereotaxic frame equipped with an anesthesia mask.
The rat was then once again placed on the start arm.
The rat was then left undisturbed for 15 min with the treated ear facing up.
A sexually receptive female rat was then introduced into the chamber.
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