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Conserved noncoding sequences, defined as sequence windows at least 100 bp long with at least 75% identity between human and rat, were selected as putative regulatory regions.
Another six sections stained with TGF- β antibody of each rat were selected, and the histological images at × 400 magnification were also obtained.
Four paraffin sections from each rat were selected to stain with hematoxylin for 5 min, differentiated by 75% hydrochloric acid ethanol for 30s, and stained by acidification eosin for 1 min.
Seven neural tissues (olfactory bulb, cortex, hippocampus, brain stem, hypothalamus, spinal cord, and dorsal root ganglion) and seven non-neural tissues (heart, lung, muscle, spleen, testicle, kidney, and liver) from each rat were selected.
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a i n (or a i h ) ∈ A1 means one RAT is selected for the call, therefore, there is no session splitting and the new (or handoff) class-i call is accepted into the selected single RAT. a i n (or a i h ) ∈ A2 means two RATs are selected for the call, therefore, there is session splitting and the new (or handoff) class-i call is split into two substreams and admitted into the selected two RATs.
Images were then reconstructed; the same coronary plane on each rat was selected for different time points and the defect area was calculated using the system software.
The Sabra salt-sensitive (SS) rat was developed using a similar strategy to that used to derive the DS rat [ 60]; however, instead of a HS diet, the SS rat was selected based on susceptibility or resistance to DOCA-salt.
The Long-Evans (Turku/AB) (LE) strain of rat was selected for these analyses because it harbours a wild-type Aryl Hydrocarbon Receptor (AHR) and is the most TCDD-sensitive rat strain reported, with an acute LD50 of 9.6 (females) or 17.7 (males) μg/kg [ 1].
The ZDF rat was selected as the test system in this study as it is an inbred rat model that, through genetic mutation and a managed diet, closely mimics human adult-onset type 2 diabetes and related complications associated with insulin resistance and β-cell failure.
In a first experiment (10 rats with body weight about 450 g), 8 rats were selected at the end of the selection period, 1 rat died during anaesthesia, and the remaining rats were treated with malonate.
In a second experiment (6 rats with a body weight of 370 g), 4 rats were selected at the end of the selection period and treated with saline instead of malonate (sham rats).
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