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Neuronal ensembles of 45 to 126 neurons per rat were recorded for 4 6 hours.
The locations of Anisakis larvae in each rat were recorded during necropsy, and all of the recovered larvae were washed with sterile PBS and placed individually into Eppendorf tubes at −20°C for further molecular analysis.
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Body weight of each rat was recorded every day during the experiment, and % change was calculated for all groups.
Light beams detected movement and the total motor activity of each rat was recorded.
Rats were permitted to recover fully within the first 7 days, and daily consumption of water and food of each rat was recorded after the 8th day.
(A) Body weight of each rat was recorded every week.
The body weight of each rat was recorded before blood was collected by cardiac puncture.
(B) high-fat diet food intake (ml/day) of each rat was recorded every day.
The mean of 10 consecutive measurements for each rat was recorded.
The mean of two consecutive responses per rat was recorded and used for data analysis.
Seizure process was recorded for 50 minutes, and the measurement from the control rat was recorded for 3 s.
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