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After two weeks, the cells from the lymph nodes of the immunized rat were fused with mouse myeloma Sp2/0-Ag14 Sp2/0-Ag14 ratio of 5:1 in 50% polyethylene glycellsMerck) solution.
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Briefly, splenocytes from immunized rats were fused with Ag8-X63 plasmocellsa cells.
The spleen cells of immunized rats were fused with myeloma cells, creating hybridoma cells.
Rat spleen cells were fused to Sp2/0 mouse myeloma cells using a standard protocol that uses polyethylene glycol-150.
However, this approach used a cell line derived from rat embryonic mesencephalon cells that were fused with mouse neuroblastoma cells as an immunogen in the guinea pig.
The SPECT and MR images were fused for rats injected with DTPA-SPION MBs (Figure 9), visualizing the concept of multimodality imaging using fusion SPECT-MRI where SPECT exclusively enables the visualization of MBs in the lungs 1 h post injection (Figure 9A) and in the liver and kidneys 24 h post injection (Figure 9B).
Murine myeloma NS1 cells were fused with spleen cells from Lewis female rats that were immunised three times.
For me, life and art are fused.
This rat sequence was fused into a β-galactosidase expression vector system.
The coding region of the ligand binding domain from the rat glucocorticoid receptor (GR) was fused to the 3'-end of the full length GI cDNA driven by the CaMV 35S promoter (35S::GI-GR).
In the rodent Cavia porcellus, as in rat and mouse, the Z2Z3 genes are fused and the A3Z1 gene is missing.
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