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Antibodies to Smad2/3 (mouse) and E-cadherin (mouse) were from BD Transduction Laboratories (San Diego, CA, USA); antibodies to phosphorylated (Ser 465/467) Smad2 (rabbit lot #21034 and #24432) were from Upstate Biotechnology (Lake Placid, NY, USA); and antibodies to ZO-1 (rat) were from Chemicon International (Temecula, CA, USA).
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SD rats were from B&K Universal Ltd (Shanghai, China).
All rats were from the vivarium of Bogomoletz Institute of Physiology.
All rats were males and all free rats were from the SD strain.
Wistar rats were from Janvier Laboratories (Le Genest St Ile, France).
Dahl salt-sensitive (SS/Jr) inbred rats were from our colony maintained at UTCOM.
All 8 rats were from the EtOH group (see Table 3).
The 36 rats were from the same stock of approximately the same age as the rats for Experiment 1.
The 30 naïve rats were from the same stock and housed in the same manner as for Experiment 1.
Wistar rat were purchased from the experimental animal center of Shandong University.
Substrains such as the JCRLA-corpulent rat were developed from these rats.
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