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Raw CEL files for human and rat were downloaded from Gene Expression Omnibus (GSE2361 [ 16], GSE952 [ 17]).
For exon-level orthology, three sets of exon sequences; human, mouse, and rat; were downloaded from USCS Genome Browser and used to generate BLAST databases.
To estimate sensitivity and specificity of variant calls, the sequence of 13 bacterial artificial chromosome (BAC) clones from various chromosomes (∼2 Mb in length) of LE/Stm rat were downloaded from the EMBL web site (accession numbers FO117624- FO117632 and FO181540- FO181543).
Validation of Genomic Variants To estimate sensitivity and specificity of variant calls, the sequence of 13 bacterial artificial chromosome (BAC) clones from various chromosomes (∼2Mb in length) of LE/Stm rat were downloaded from the EMBL web site (accession numbers FO117624- FO117632 and FO181540- FO181543).
The genome sequences and annotations of four mammals (human, cow, mouse and rat) were downloaded from the Ensembl Web site (http://www.ensembl.org), in March 2009.
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The file containing the whole pathways of rat was downloaded from the KEGG database (http://www.genome.jp/kegg/pathway.html).html
Raw expression data for 11 tissues in mouse and rat was downloaded from the GNF BioGPS system [ 33].
Pesudogenes corresponding to human, mouse and rat genome were downloaded from http://www.pseudogene.org[ 42].
The unigenes of the blind mole rat unigenes were downloaded from GenBank under the accession number provided by [ 15].
Next, the peaks (hypermethylated regions) were identified using MACS software and the rat CGIs were downloaded from the UCSC database (http://genome.ucsc.edu).ucsc.edu
Chromosomal positions corresponding to genes, introns, CDS, 5/UTR, 3/UTR, for human, mouse and rat genome were downloaded from UCSC genome browser [ 32].
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