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Based on an average rat weight of 300 g, this would be equivalent to the consumption of about 1000 ml of OPP by a 60 kg human subject, which is calculated based on the body surface area normalisation method.
Using 166 g as the daily dose of dried herbs for an adult, the relative body surface-area ratio of rats to humans of 7 1, adult human weight of 60 kg and rat weight of 0.2 kg, the daily dose of dried herbs for one rat was calculated as 3.87 g = 166 g*7*(0.2 kg/60 kg), and that of Premarin as 0.015 mg/rat.
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After four weeks exhaustive exercise, the rats weight of control and EP+EPO groups was increased much more than those of rats in ET group (P < 0.05).
Rat weights at the time of experiments were 250 to 300 g.
Healthy female Wistar rats (body weight of 200 250 g at start of experiments) were used.
Feed taken by the rats and weight of the animals were controlled at the end of the study.
In rats, absolute weight of thymus is maximal in 2-month-old animals and then strongly lowers with age down to 10-15% of maximal value at 14 months.
The amount administered was at a concentration of 10 mg/kg rat based on the weight of the rat at day 25.
Similarly, in honey-treated rats, the weights of the vagina also showed significant increased values when compared to rats in OVX group.
The validated method has been successfully applied to quantify SF concentrations into a single dose (4 mg/200 g body weight of rat) in rats.
Male Sprague Dawley rats with average weight of 170 180 g were obtained from the Animal House of University of Port Harcourt, Rivers State Nigeria.
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