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The injections were performed once daily for 10 days based on rat weight and clinical protocol.
On day 21 (weaning day), pups were anaesthetized with ketamine/xylazine (ketamine 90 mg/kg plus xylacine 10 mg/kg of rat weight) and sacrificed by humanitarian methods.
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The rat weights and feed intakes were recorded during experiments.
Rat weights and lengths were measured weekly.
In addition, we analyzed the differences among neonate rat weights and heights at birth, and these parameters indicated a decrease (P < 0.0001) in the three raltegravir groups compared with the negative control group (Table 2).
Results showed that rats' weight and sucrose intake were significantly lower in stressed group than those in control group.
Results showed that rats' weight and sucrose consumption were significantly lower in stressed group than those in control group, while the last time of sucrose consumption was improved by memantine.
Data were expressed as mean ± SEM in the rat weight changes and others were expressed as mean ± SD.
Data were expressed as mean ± SEM in the rat weight changes and impaired glucose tolerances by OGTT methods, others were expressed as mean ± SD.
Rats were anesthetized with isoflurane (1 mL/100 g rat weight), beheaded, and the brain removed.
Using 166 g as the daily dose of dried herbs for an adult, the relative body surface-area ratio of rats to humans of 7 1, adult human weight of 60 kg and rat weight of 0.2 kg, the daily dose of dried herbs for one rat was calculated as 3.87 g = 166 g*7*(0.2 kg/60 kg), and that of Premarin as 0.015 mg/rat.
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