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Each rat was perfused through the heart with 50 ml 1% sodium nitrate, followed by 500 ml 4% paraformaldehyde in 0.1 M phosphate buffer (PB).
The rat was perfused 1 week after infusion of tracer.
Each rat was perfused with saline followed by 4% paraformaldehyde.
Each rat was perfused with citrate-buffered 1% paraformaldehyde for 5 min to clear the dye.
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Pups were born by natural delivery and reared until P16, and then 3 pups from each mother rat were perfused as described above.
The mesenteric vascular beds without an endothelium isolated from male Wistar rats were perfused with Krebs solution and perfusion pressure was measured.
Kidneys from Sprague-Dawley rats were perfused for 180 min with constant perfusion pressures (80 mmHg (n = 4); 120 mmHg (n = 4)) in a closed circuit system.
After preliminary perfusion with mKRB for 5 min, pregnant rats were perfused with mKRB containing 2 μM BPA-GA for 20 min. Then, mKRB without BPA-GA was perfused for 70 min (a total of 90 min).
Isolated kidneys from Sprague-Dawley rats were perfused for 180 min with a pressure of 100 mmHg in a recirculating perfusion system.
At 22 days post-injury, rats were perfused and brains extracted and processed for lesion size.
Rats were perfused with 4% paraformaldehyde, spleens removed and cryoprotected, and 50-microm floating sections were cut on a freezing microtome.
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