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Every rat was kept in its own cage.
After radiotracer injection, the rat was kept warm for a 1-h uptake period until imaging and then placed in the imaging bed in the prone position, sedated, with the tumor on its back positioned upright.
The rat was kept under anesthesia with 1.5% (v/v) isoflurane during the scan, and its body temperature was maintained with a 40°C water circulation system (T/Pump TP401, Gaymar Industries, Orchard Park, NY, USA).
Each rat was kept in a separate cage under 12-hours dark-light cycles with free access to food and water.
The scalp wound was closed with surgical clips, and the rat was kept in a warm environment until resuming normal activity.
After being placed on a swing, each rat was kept stationary for 7.5 hours per day between 9 00 AM and 5 00 PM.
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Serum samples before and after resin adsorption therapy (RAT) were kept at −80 °C for ROS testing and transwell migration assay.
The weight of all rats was kept at 80% of free-feeding body weight.
The first group (10 rats) was kept as control and received distilled water.
An age-matched control group of rats was kept in room air (normoxia, n = 6).
The core body temperature of the rats was kept at 37.5 ± 0.5°C and continuously monitored via a rectal sensor.
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