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SD rats were injected STS-Nano-RBC or STS via tail vein, each rat was injected with approximately 1.0 mg of STS injection or STS-Nano-RBC.
The scanning was started during injection into the first rat; the second rat was injected 30 s later.
Three days later, each rat was injected with a lethal bolus dose of LPS (15 mg/kg) and had its colonic temperature recorded.
Twenty-four hours before the PET studies, turpentine oil (Sigma-Aldrich; 0.05 ml per rat) was injected subcutaneously into their neck area in order to induce a sterile inflammation [10].
Following baseline testing on Day 8, the rat was injected with 10 μL of 1% AITC, 10% AITC, saline, or mineral oil onto the dura mater using an injection cannula inserted into the guide cannula.
Each rat was injected intravenously twice through the caudal vein, once a week apart, with 5 μg/kg body weight of bryostatin-1 (Jiangbo, Xuying, Yuping, Xili, Yiwen, et al., 2010).
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One of the six rats was injected in the forefoot with the PCSN probe solution; these results were not analyzed.
Serum from these rats was injected into individual rats, which were tested for seroconversion.
†Serum from wild rats was injected into laboratory rats B76 and B84.
A control group of rats was injected with sterile physiological saline in the left ear.
Baby rats were injected with incubated viruses and monitored for signs of illness.
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