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Each rat was implanted with a head assembly containing 18 individually drivable tetrodes [60] aimed at the medial prefrontal cortex (infralimbic and prelimbic cortex).
Each rat was implanted with two microdialysis probes placed bilaterally in the cortex, with one probe serving as a control (hypoosmotic solution only) and the probe on the other side (chosen at random) serving as the experimental condition (hypoosmotic solution plus drug).
Each rat was implanted with a miniature microdrive with two tetrodes aimed at pre- or parasubiculum.
Immediately after IR, each rat was implanted with a single Alzet 2004 infusion pump for delivery of 0.25 μl hr−1 of CCX662 solution (six rats) or vehicle (six rats) for 4 weeks.
Each rat was implanted subcutaneously by two sterilized PU and CPU3 membranes (the same size as 1 well of 96-well culture plate) after the rat was given an analgesic (ketoprofen 1.5 mg) and anesthetized with 5% chloral hydrate (intraperitoneal injection, 6 mg/kg).
Eight rats were implanted with CPEs into the dHPC of one hemisphere and the vHPC of the other hemisphere (right dHPC/left vHPC n=4; left dHPC/right vHPC n=4), and one rat was implanted with CPEs into the dHPC of both hemispheres.
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To evaluate the inhibitory effect of the selective kisspeptin antagonist on pulsatile LH secretion, a group of rats was implanted with an icv guide cannula (22 gauge; Plastics One, Roanoke, VA, USA) directed towards the left lateral ventricle, the co-ordinates for implantation being 0.6 mm lateral and 1.5 mm posterior to bregma, and 4.5 mm below the surface of the dura.
To identify the neural site for kisspeptin-GPR54 signalling to control pulsatile LH secretion, a separate group of rats was implanted with bilateral guide cannulae (22-gauge; Plastics One) directed at the GnRH-rich areas of the mPOA or the ARC for microinfusion of kisspeptin or its antagonist.
A group of naive rats was implanted with guide cannulae and received an intracerebral microinjection of 2 microliters of ACSF and served as a control of the intracerebral drug microinjection.
To establish ANG II-induced hypertension, 7 days after the implantation of a radio-transmitter into the abdominal aorta, male Wistar rats were implanted with an ANG II-containing Alzet osmotic pumps subcutaneously, which delivered at a rate of 200 or 50 ng/kg/min.
The rats were implanted with a tiny array of electrodes, which threaded from the top of the head down into two neighboring pieces of the hippocampus, a structure that is crucial for forming these new memories, in rats as in humans.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com