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pCMVTRAK2 (rat) was generated as in [6]; recombinant TRAK2 has a C-terminal Flag-tag.
mEmerald and Fusion Red-tagged F-tractin, an acting binding peptide derived from the Itpka protein from rat was generated by cloning into a Clontech-style vector containing the advanced EGFP variant Emerald (F64L, S65T, S72A, N149K, M153T, I167T, A206K).
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Orthologous gene clusters of human, mouse and rat were generated from the OMA database [ 36].
Adult retinoid-deficient rats were generated by a vitamin A-free diet from weaning in order to allow a normal development.
FSL/FRL rats were generated as a result of selective breeding of out-bred Sprague-Dawley rats for differences in the effects of the anticholinesterase agent diisopropylfluorophosphate (DFP) [23].
DA.F344(congenicongenic rats were generated as previously described [ 9].
GLO1 transgenic (GLO1 Tg) rats were generated as described previously (Inagi et al., 2002).
The trpc4-KO rats were generated using the Sleeping Beauty transposon system.
Double transgenic rats were generated by breeding the response lines with driver lines, generating animals heterozygous for both transgenic alleles.
HS rats are generated by intercrossing several inbred progenitor strains followed by cross breeding for more than 50 generations.
To investigate the correlation between postmenopausal osteoporosis (PMO) and the pathogenesis of periodontitis, ovariectomized rats were generated and the experimental periodontitis was induced using a silk ligature.
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