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The rat was fixed in a specially designed cabin with an adjustable aperture showing the frontal, parietal, and occipital parts of the head exposed to explosion; the eyes, ears, mouth, and nose were protected by the cabin.
The rat was fixed to a stereotaxic apparatus (David Kopf, Tujunga, CA, USA) with the incisor bar set 3.3 mm ventral to interaural line.
Before the MCAo operation, the head of each anesthetized rat was fixed to a stereotactic frame.
Another piece of heart from each rat was fixed in 4% paraformaldehyde and embedded in paraffin.
The left kidney from each rat was fixed in 10% phosphate-buffered formalin for morphologic analyses.
One kidney from each rat was fixed in buffered formalin, embedded in paraffin and used for immunohistochemistry.
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Brains for E16 or P6 Sprague Dawley (SD) rat were fixed with 4% paraformaldehyde, followed by addition with 10% sucrose.
The four limbs of the rat were fixed supinely after it was anesthetized and the two-dimensional ultrasonography was performed after penis was fully exposed.
Liver samples from each rat were fixed in 10% (volume/volume) formaldehyde, embedded in paraffin and stained with hematoxylin-eosin (HE).
Liver tissues from the right lobe of the liver of each rat were fixed in 4 % buffered paraformaldehyde, and dehydrated in a graded alcohol series.
Liver tissue samples from each rat were fixed in Bouin's solution for 24 h, then dehydrated in a graded series of isopropyl alcohol, and embedded in paraffin blocks.
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