The chopped lung tissue from a single rat was digested in 20 mL of HEPES-buffered solution (145 mM NaCl, 5 mM KCl, 1 mM CaCl2, 505 mM glucose, and 10 mM HEPES, pH 7.4), containing collagenase (0.1%), elastase (40 U/mL), bovine serum albumin (0.5%), and DNAse (0.018%) in a shaker water bath for 30 min at 37°C.
The chopped lung tissue from a single rat was digested in 20 mL HEPES-buffered solution (145 mM NaCl, 5 mM KCl, 1 mM CaCl2, 5.5 mM glucose, and 10 mM HEPES, pH 7.4) containing collagenase (0.1%), elastase (40 U/mL), bovine serum albumin (0.5%), and DNAse (0.018%) in a shaker water bath for 30 min at 37°C.
One μg of genomic DNA from the L4 DRG of each of 5 adult male Sprague Dawley rats was digested with MspI.
Mastication works, for experiments with tame pythons show that pureed rats are digested more effectively than those in their native state.
For primary oligodendrocyte cultures, dissected cortices of newborn mice or rats were digested in 0.25 mg ml−1 Trypsin/EDTA for 10 min followed by triturating and plating in plating media (DMEM 4.5 g l−1 Glucose, 10% fetal calf serum containing about 300 μg ml−1 cholesterol, GlutaMAX, penicillin/streptomycin).
Muscles from young and old rats were digested for 90 or 120 minutes, respectively, for optimal recovery of intact myofibers.
Briefly, DRG from 3-week-old SD rats were digested with trypsin/EDTA solution for 40 min and dissociated in growth medium containing DMEM, 5% inactivated fetal bovine serum, 5 mM glutamine, B27 supplement (GIBCO), 100 ng/ml of nerve growth factor (Becton Dickinson) and 0.6% dextrose.
The lungs of exposed rats were digested to quantify dose, fiber dimensional changes over time, and clearance kinetics.
Cartilage slices from knee joints of rats were digested with 0.05% trypsin and 0.53 mM EDTA (Gibco Invitrogen) at 37°C for 30 min, followed by 0.3 mg/mL collagenase P (Roche Diagnostics GmbH, Mannheim, Germany) at 37°C for 4 h.
Briefly, ventricles from 1- to 3-day-old GFP-positive Wistar neonatal rats were digested at 37°C in Hank's balanced salt solution containing collagenase (Worthington Biochemical Corporation, Lakewood, NJ, USA).
To generate the proteolytically processed form, recombinant rat TRAP was digested at 37°C for 40 minutes with recombinant human cathepsin K (kindly provided by Dr. Robert Dodds) in 5mM NaAc pH 5.5, 1 mM EDTA and 10 mM DTT using a 1∶1 molar ratio.
