Exact(3)
Immediately after the rat was decapitated, the heart was removed and placed in diethylpyrocarbonate (DEPC) treated water.
Briefly, after anaesthetization using ether, the rat was decapitated and the brain was removed quickly and immersed in carbogenated ice-cold Gey's solution (composition in mM: 130 NaCl, 4.9 KCl, 1.5 CaCl2·2H2O, 0.3 MgSO4·7H2O, 11 MgCl2·6H2O, 0.23 KH2PO4, 0.8 Na2HPO4, 5 glucose, 25 HEPES, 22 NaHCO3, pH 7.32).
To analyse tissue distribution of AP-5, a mouse or rat was decapitated and tissues were harvested and homogenized with a dounce homogenizer in lysis buffer (10 m m Tris HCl pH 7.4, 100 m m NaCl, 1 m m EGTA, 2 m m MgCl2, 1% sodium dodecyl sulphate) containing complete protease inhibitor (Roche) and 10 U/ml Benzonase (Novagen).
Similar(57)
P7 rats (Wistar rat) were decapitated and their brains were dissected, then washed in phosphate buffered saline (PBS) briefly, and immersed in 4% paraformaldehyde at 4°C overnight.
For thalamic cultures, P1 mouse or rat pups (CD-1 mouse or Sprague Dawley rat) were decapitated and their thalami were dissected.
A group of awake, conscious rats was decapitated while the EEG was recorded before, during and after the severing of the neck.
Rats were decapitated either 3 or 24 h after burn injury.
At the end of the treatment period, all rats were decapitated.
After the behavioural test, the rats were decapitated for Western blot analysis.
Rats were decapitated either 3 or 24 hours after burn injury.
Rats were decapitated either 3 h or 24 h after burn injury.
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