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Every sixth 40-μm-thick section of each SN of every rat was counted (from 8to1010 sections per animal, usually nine sections per rat).
The sections were stained with anti-CS antiserum and the number of LS in six sections from each rat was counted.
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After completion of the study, the baby rats were counted and separated by sex with observation for abnormality at the time of weaning.
The morphologically undamaged MNs (i.e. large neurons, with a soma diameter >20 μm and a distinguishable nucleus, similar in appearance to those of the contralateral ventral horn and to those in control rats) were counted in a 10× microscopic field.
The number of blood vessels that were identified as positive isolectin B4, the marker for rat endothelial cells, was counted at × 40 magnification (n = 5 for each group).
The number of pERK-positive axo-spinous synaptic contacts in layers I and II/III of the rat primary visual cortex was counted in three distinct animals for each of the four different visual stimulation conditions (DR, DR + 2.5 min of light exposure, DR + 15 min and DR + 40 min).
For all the quantifications an average number of 3 sections was counted per rat spanning about 500 µm along the antero-posterior axis.
The number of times the rat exhibited guarding, licking and shaking was counted and the total duration of this behavior was measured over 5 min immediately after intraplantar administration of capsaicin (2 mM).
The number of rotations was counted visually for each rat.
An arm entry was counted when all four limbs of the rat were within an arm.
As it is not known if these rat loci contain unique sub-loci, human risk associated polymorphisms mapping to overlapping rat regions were counted only once.
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