However, over-expression of TFF3 in a RIN cell line and rat islets using recombinant adenovirus lead to an increased proliferation which can be arrested by knockdown of TFF3 using siRNA.
The 2A peptide was first shown to work in vivo in the rat brain, using recombinant adeno-associated viruses containing 2A dependent vectors [ 9].
We report here that we have successfully generated four Bcl-G-specific rat monoclonal antibodies using recombinant full-length mBcl-G as the immunogen.
We investigated this further using recombinant rat neurofascin-155 (rrNfasc155) in attempts to absorb demyelinating and axopathic components of the autoantibody repertoire.
This possibility is supported by the recent identification of autoantibodies against p62 using recombinant rat or human protein in 50% of PBC sera by immunoprecipitation of S-radioactively labeled p62 and in 40% by immunoblotting [ 26].
Then, to confirm that loss of Par-4 cleavage was a direct consequence of CK2 phosphorylation and not an artifact introduced by the mutation, we performed an in vitro kinase assay on rat GST-Par-4 using recombinant CK2, followed by an in vitro caspase assay using recombinant caspase 3.
To compare embryo implantation in Wistar rats submitted to ovarian stimulation using recombinant FSH (rFSH) with cetrorelix acetate or leuprolide acetate.
Several groups have studied the role of phosphorylation at the S129 in α-synuclein in a rat model for Parkinson's disease using recombinant adeno-associated viral (rAAV) vectors.
Assays were developed and standardized using recombinant proteins from expression of rat-WT-TyrH and rat-Δ117PheH.
In this review we present our results and experiences in performing gene therapy of cerebral stroke using recombinant adeno-associated virus (rAAV) vectors in a rat model.
