Blood samples were collected from the fossa orbitalis wein of rats under light ether anesthesia into micro-fuge tubes containing heparin as an anticoagulant at 2, 5, 15, 30, 45, 60, 90, 120, 240, 360, 600 min post-dosing and then centrifuged at 3500 r/min for 15 min at 4 °C.
At time 0 minutes, blood collection was performed in 8-hour-fasted obese ZSF1 rats under light isoflurane anesthesia from the sublingual vein.
On day 29, blood was collected from the retroorbital plexus of overnight fasted rats under light ether anaesthesia and serum was separated by centrifugation at 3000 rpm for 15 min.
Blood samples (0.5 ml) were collected from the retro-orbital plexus of rats under light ether anaesthesia into heparinized tubes at 0, 1, 3, 5, 10, 20, 30, 60, 90, 120, and 240 min after dosing with 5-FU.
Interestingly, putatively enkephalinergic IGL neurons generate action potentials with an infra-slow oscillatory (ISO) pattern in vivo in urethane anesthetised Wistar rats, under light-on conditions only.
We could also elicit movements of the whiskers from a lateral region which largely overlapped the somatosensory whisker region in two of the rats mapped under light anaesthesia (not shown), but not in the rats under deep anaesthesia.
This location was similar to that in the rats mapped under light anaesthesia.
Experiments were carried out in adult male Wistar rats, kept under light between 0800 and 2000 h daily.
Thus the distribution of the nature of movements evoked was not different from that in rats mapped under light anaesthesia.
As for the rats mapped under light anaesthesia, the area of the caudal forelimb region was larger than that of the rostral forelimb region.
