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The distribution of DOR mRNA in human and rat tissues was examined by Northern blot.
Moreover, the abundance of mRNA between mouse and rat tissues was similar, but these patterns were quite different from the results obtained from human tissues.
One half of each group of rat tissues was fixed and embedded in OCT compound (Miles Scientific) and the other half snap-frozen in liquid nitrogen for fluorescence microscopy and alkaline phosphatase staining for capillary count.
The concentration of auraptene in rat tissues was analyzed by reverse phase HPLC.
Total RNA from HeLa cells and rat tissues was extracted using the TRIzol reagent (Invitrogen) according to the manufacturer's recommendations.
The estimation of DA, NE, and 5-HT levels in the selected rat tissues was carried out according to the fluorometric method described by Ciarlone [ 19].
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Mouse and rat tissues were purchased from Biochain (Hayward, California) and Clontech.
Nuclear membrane area of cell from different rat tissues were calculated on the basis of the total phospholipidic content.
Acute collection of X. laevis and rat tissues were performed according to our laboratory protocol (#AN080281-01C) using animals obtained from our frog and rodent colonies, and all procedures for animal husbandry and euthanasia were approved by the UCSF Institutional Animal Care and Use Committee.
Nuclei from one gram of five different rat tissues were de-shielded with 50 mM NaCl, washed three times with 10 ml of 0.25 M sucrose and resuspended in 0.25 M sucrose (10 ml).
Rat tissues were processed as described for immunohistochemical analyses.
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